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Gibco™ RPMI 1640 培养基(ATCC 改良)

简要描述:Gibco™ RPMI 1640 培养基(ATCC 改良)
RPMI 1640培养基初开发用于悬浮和单层培养人类白血病细胞。此后,罗斯威尔公园纪念学院(RPMI)1640培养基被发现适用于多种哺乳动物细胞,包括HeLa,Jurkat,MCF-7,PC12,PBMC,星形胶质细胞和癌。我们为各种细胞培养应用提供了多种RPMI 1640培养基改良剂。

  • 产品型号:A1049101
  • 厂商性质:经销商
  • 更新时间:2024-08-22
  • 访  问  量:1237

详细介绍

品牌Thermofisher Scientific/赛默飞世尔货号A1049101
规格500ml供货周期现货
主要用途哺乳动物细胞培养应用领域生物产业

Gibco™ RPMI 1640 培养基(ATCC 改良)相关介绍:

RPMI 1640 Medium was originally developed for the suspension culture of human leukemic monolayer cells. Roswell Park Memorial Institute (RPMI) 1640 Medium has been found to be suitable for a variety of mammalian cells including HeLa cells, Jurkat cells, MCF-7 cells, PC12 cells, PBMC cells, astrocytes, and cancer cells. For a wide range of cell culture applications, we offer RPMI 1640 Modified Medium in various components. Use the media selection tool to find suitable formulations.

This RPMI is modified as follows:

Include

L-Glutamine

HEPES:

Phenol red

sodium pyruvate

high sugar

low sodium bicarbonate

Full formulations are available


RPMI 1640 medium is unique compared to other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B 12 and PABA, which are not available in Eagle's MEM and DMEM. In addition, the medium contains high concentrations of the vitamins inositol and choline. This RPMI formula has been modified by ATCC to contain high concentrations of dextrose, low sodium bicarbonate, sodium pyruvate, HEPES and low L-glutamine. RPMI 1640 Medium does not contain proteins, lipids or growth factors. Therefore, RPMI 1640 medium requires supplements, usually 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (1.5 g/L), thus requiring an environment of 5–7% CO 2 to maintain physiological pH.

cGMP Manufacturing and Quality System:

RPMI 1640 Medium is manufactured in a cGMP compliant facility located in Grand Island, NY. The facility is an FDA registered medical device manufacturer and is ISO 13485 certified.

本公司提供Gibco™ RPMI 1640 培养基(ATCC 改良),如有需要欢迎来咨询!



Specification

cell line                      

HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes and cancer cells                        

cell type                        

leukemia cells                        

Classification                        

No animal origin                        

concentrate                        

1X                        

form                        

liquid                        

product type                        

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)                        

Serum Level                        

Standard serum added                        

validity period                        

12 months from the date of manufacture                        

sterile                        

sterile filtration                        

with additives                        

High Glucose, Glutamine, HEPES:, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate Low                        

Environmental protection function                        

Green Sustainable Packaging                        

product line                        

Gibco™                        

Shipping conditions                      

room temperature                        

Content and Storage

Storage Conditions: 2 – 8°C. Avoid light.
Transport conditions: room temperature
Validity period:
12 months from the date of production                        






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